Enzyme efficiency but not thermostability drives cefotaxime resistance evolution in TEM-1 β-lactamase
نویسندگان
چکیده
منابع مشابه
Enzyme Efficiency but Not Thermostability Drives Cefotaxime Resistance Evolution in TEM-1 β-Lactamase
A leading intellectual challenge in evolutionary genetics is to identify the specific phenotypes that drive adaptation. Enzymes offer a particularly promising opportunity to pursue this question, because many enzymes' contributions to organismal fitness depend on a comparatively small number of experimentally accessible properties. Moreover, on first principles the demands of enzyme thermostabi...
متن کاملTEM & SHV genes in extended spectrum β-lactamase producing Klebsiella species & their antimicrobial resistance pattern
Background & objectives: Extended spectrum β-lactamases (ESBLs) are often plasmid mediated derived from mutations in the classic TEM and SHV genes by one or more amino acid substitution around the active site. Detection of TEM and SHV genes by molecular methods in ESBL producing bacteria and their pattern of antimicrobial resistance can provide useful information about its epidemiology and risk...
متن کاملA Fitness Cost Associated with the Antibiotic Resistance Enzyme SME-1 β-lactamase
The blaTEM-1 β-lactamase gene has become widespread due to the selective pressure of β-lactam use and its stable maintenance on transferable DNA elements. In contrast, blaSME-1 is rarely isolated and is confined to the chromosome of carbapenem resistant Serratia marcescens strains. Dissemination of blaSME-1 via transfer to a mobile DNA element could hinder the use of carbapenems. In this study,...
متن کاملDeactivation of TEM-1 β-Lactamase Investigated by Isothermal Batch and Non-Isothermal Continuous Enzyme Membrane Reactor Methods.
The thermal deactivation of TEM-1 β-lactamase was examined using two experimental techniques: a series of isothermal batch assays and a single, continuous, non-isothermal assay in an enzyme membrane reactor (EMR). The isothermal batch-mode technique was coupled with the three-state "Equilibrium Model" of enzyme deactivation, while the results of the EMR experiment were fitted to a four-state "m...
متن کاملTEM-1 β-lactamase as a source of resistance to sulbactam in clinical strains of Acinetobacter baumannii.
OBJECTIVES Sulbactam is well known to have clinically relevant intrinsic activity against Acinetobacter baumannii. Although secondary resistance to this drug has long been reported in acinetobacters, virtually nothing is known about its molecular basis. The aim of this study was to test the hypothesis that β-lactamase TEM-1 is responsible for sulbactam resistance in A. baumannii. METHODS Seve...
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ژورنال
عنوان ژورنال: Molecular Biology and Evolution
سال: 2017
ISSN: 0737-4038,1537-1719
DOI: 10.1093/molbev/msx053